1. Process of introducing new laboratory method in routine use

2. Repeatability, reproducibility, intermediate precision and interlaboratory precision

3. Measurement of within run precision using 20 sets of within-batch-duplicate measurement

4. Precision profile of an examination at different analyte concentration

5. Explain repeatability and reproducibility with examples

6. Evaluation of the Linearity of Quantitative Measurement Procedures CLSI EP 06-A

7. LoB,LoD,LoQ and LoL

8. Procedures for setting analytical goals for clinical chemistry examinations

9. Bland Altman plot for comparison between two methods

10. Absolute and relative Bland Altman plot for comparison between two methods

11. Regression analysis

12. Concept of significant digits in clinical chemistry

13. Traceability and measurement uncertainty

14. Sensitivity and specificity of diagnostic test and ROC curve

15. Types of clinical questions addressed in laboratory medicine with examples

16. Hierarchy of evidences for decision making about appropriateness of a laboratory examination

17. Standards for reporting diagnostic accuracy (STARD) used for evaluating evidence published for accuracy of 
laboratory test

18. Critical appraisal and systemic review of diagnostic tests

19. Economic evaluation of diagnostic tests

20. Different meanings of “Normal Value” and difference from “Reference value” and “Clinical decision limits”

21. Conditions to compare patient results with reference values

22. Strategies for selecting reference individuals for determining reference values of a diagnostic test

23. Parametric and nonparametric methods for determining reference values

24. Univariate, multiple univariate and multivariate reference values

25. Subject based reference values

26. Circadian rhythm as preanalytical variable

27. Diet and alcohol as preanalytical variable

28. Noncontrollable preanalytical variable

29. Biological variability of clinical chemistry examinations

30. Recommended order of draw, stopper color, content and inversions

31. Types of vacuum tubes for collection of blood for serum

32. Newer advances in vacuum tubes technology for patient and phlebotomist safety

33. Skin puncture for sample collection

34. Arterial puncture for sample collection

35. Various anticoagulents and preservatives in blood

36. Collection of urine specimen

37. Cost of Quality with reference to clinical laboratory

38. Concept of six sigma and measurement of sigma in a clinical chemistry laboratory

39. Calibrator traceability

40. LJ Chart for quality control

41. Westgard rules for interpretation of QC results

42. Control of quality using patient data from single and multiple patients

43. Desirable characteristics of EQA program for clinical chemistry

44. Explain Trueness, accuracy and precision with examples

45. Causes and prevention of error and hazards in sample collection

46. mole, molarity, molality and normality

47. Concentration quantities and units in clinical biochemistry

48. SI units in clinical biochemistry

49. Standardized reporting of test results

50. Reagent grade water – CLSI specifications and preparation/instrumentation

51. Reference materials, Reference methods and Reference laboratories

52. Measurement of volume in clinical chemistry

53. Micropipette/Pipette/ Volumetric flask calibration

54. Types, operating principles, calibration, care and maintenance of centrifuges

55. Buffers in clinical chemistry reagents – principles, preparation and uses

56. Ethical issues in clinical chemistry

57. Hazards in clinical laboratory

58. Classification of fires and fire extinguisher requirements

59. Color coding, order of draw, mixing recommendations of vacuum tubes for sample collection