1.How H2O2 is formed in human body? Describe biochemical reactions causing H2O2 mediated damage to cell membrane lipid. How NADPH is used to metabolize H2O2? How G6PD mutations are inherited| What are biochemical events causing hemolysis in G6PD deficiency? Explain principle of Methelene Blue based screening test for diagnosis of G6PD deficiency.
2.Explain role of Fe2+, heme and globins in hemoglobin mediated O2 and CO2 transport. Emphasize role of distal and proximal histidine of globin. Enlist series of molecular events responsible for increased O2 affinity of Hb when one of its globin bind O2. Explain molecular basis of relationship between O2 and carbon monoxide in context of hemoglobin structure and function. What is carbon monoxide concentration in various environment?
3.Enzymatic pathways of methemoglobin reduction and biochemical basis for use of methelene blue in treatment of methemoglobinemia.
4.Enzymatic pathways of methemoglobin reduction and biochemical basis for use of methelene blue in reagents for diagnosis of G6PD deficiency.
5.Amino acid substitutions in hemoglobin variants and correlation with electrophoretic mobility and sickling test
6. Cyanide poisoning – biochemical consequences and biochemical basis of its treatment
7. Biochemical cause of major signs and symptoms of porphyrias
8. Biochemical basis of etiology, clinical features, diagnosis and treatment of unconjugated neonatal hyperbilirubinemia.
9. beta thalassemia – genetics, pathogenesis, diagnosis