1. Process of introducing new laboratory method in routine use
2. Repeatability, reproducibility, intermediate precision and interlaboratory precision
3. Measurement of within run precision using 20 sets of within-batch-duplicate measurement
4. Precision profile of an examination at different analyte concentration
5. Explain repeatability and reproducibility with examples
6. Evaluation of the Linearity of Quantitative Measurement Procedures CLSI EP 06-A
7. LoB,LoD,LoQ and LoL
8. Procedures for setting analytical goals for clinical chemistry examinations
9. Bland Altman plot for comparison between two methods
10. Absolute and relative Bland Altman plot for comparison between two methods
11. Regression analysis
12. Concept of significant digits in clinical chemistry
13. Traceability and measurement uncertainty
14. Sensitivity and specificity of diagnostic test and ROC curve
15. Types of clinical questions addressed in laboratory medicine with examples
16. Hierarchy of evidences for decision making about appropriateness of a laboratory examination
17. Standards for reporting diagnostic accuracy (STARD) used for evaluating evidence published for accuracy of laboratory test
18. Critical appraisal and systemic review of diagnostic tests
19. Economic evaluation of diagnostic tests
20. Different meanings of “Normal Value” and difference from “Reference value” and “Clinical decision limits”
21. Conditions to compare patient results with reference values
22. Strategies for selecting reference individuals for determining reference values of a diagnostic test
23. Parametric and nonparametric methods for determining reference values
24. Univariate, multiple univariate and multivariate reference values
25. Subject based reference values
26. Circadian rhythm as preanalytical variable
27. Diet and alcohol as preanalytical variable
28. Noncontrollable preanalytical variable
29. Biological variability of clinical chemistry examinations
30. Recommended order of draw, stopper color, content and inversions
31. Types of vacuum tubes for collection of blood for serum
32. Newer advances in vacuum tubes technology for patient and phlebotomist safety
33. Skin puncture for sample collection
34. Arterial puncture for sample collection
35. Various anticoagulents and preservatives in blood
36. Collection of urine specimen
37. Cost of Quality with reference to clinical laboratory
38. Concept of six sigma and measurement of sigma in a clinical chemistry laboratory
39. Calibrator traceability
40. LJ Chart for quality control
41. Westgard rules for interpretation of QC results
42. Control of quality using patient data from single and multiple patients
43. Desirable characteristics of EQA program for clinical chemistry
44. Explain Trueness, accuracy and precision with examples
45. Causes and prevention of error and hazards in sample collection
46. mole, molarity, molality and normality
47. Concentration quantities and units in clinical biochemistry
48. SI units in clinical biochemistry
49. Standardized reporting of test results
50. Reagent grade water – CLSI specifications and preparation/instrumentation
51. Reference materials, Reference methods and Reference laboratories
52. Measurement of volume in clinical chemistry
53. Micropipette/Pipette/ Volumetric flask calibration
54. Types, operating principles, calibration, care and maintenance of centrifuges
55. Buffers in clinical chemistry reagents – principles, preparation and uses
56. Ethical issues in clinical chemistry
57. Hazards in clinical laboratory
58. Classification of fires and fire extinguisher requirements
59. Color coding, order of draw, mixing recommendations of vacuum tubes for sample collection